Design and validation of a survey on antimicrobial use and resistance in Chile
Published 2026-07-09
How to Cite
Copyright (c) 2026 QF Francisca Aldunate, Alejandra Vaquero Orellana, Carmen Gloria Lobos Saldías, Natalia Santis Alay, Mario Soto Marchant, Constanza Coros Fuentes, Juan Otárola Navarrete

This work is licensed under a Creative Commons Attribution 4.0 International License.
Abstract
Background: Antimicrobial resistance (AMR) is a growing threat to public health, driven by the inappropriate and excessive use of antimicrobials. Despite the existence of international and national plans, significant knowledge gaps remain among the general public, highlighting the need to strengthen awareness and advocate for the careful use of antimicrobials. Aim: To validate a tool for assessing the Chilean adult population’s knowledge and behavior concerning antimicrobial use and AMR, aimed at informing future health communication strategies. Methods: A cross-sectional observational study conducted in 2023 comprised three stages: survey design based on a literature review; content validation by experts and public health system users, employing CVR and CVI; and a pilot test involving 224 participants, using psychometric analyses to assess internal consistency and reliability, in conjunction with a statistical analysis in RStudio. Results: The process yielded a questionnaire validated in three phases, resulting in 27 items distributed across four dimensions, with adequate item-total correlations and acceptable internal consistency (Cronbach’s alpha = 0.76; Omega = 0.86). Conclusion: A robust tool was developed to assess the knowledge and use of antimicrobials, as well as awareness of AMR among public health system users
Keywords: knowledge; questionnaire; Drug Resistance, Microbial; Drug Utilization¸ Anti-Infective Agents, awareness.
